Decrease in the number of bacteria for nucleic acid extraction and sampling of microbiome from the environment

DOI: 10.4225/50/58106ee2a9961
22 November 2016

Purpose / Context

Public facilities such as homes for aged individuals and schools are considered at an increased risk for the spread of infections. To our knowledge, there has been no research on the relationship between the microbiome and human activity at such places. Therefore, we investigated the microbiome of locations housing individuals with weakened immune systems due to the spread of infection, to reveal its characteristics. We attempted to establish a method to sample microbiomes from these environments.

Methodology

For ease of sampling, we used Lactobacillus instead of the microbiome directly. We dropped the diluted Lactobacillus onto a plate and swabbed it. After DNA extraction from these bacteria, we calculated the number of bacteria by real-time PCR analysis.

Results

Results showed that approximately 50% of bacteria were lost during sampling and nucleic acid extraction. In this experiment, we found a faint band for the negative control by gel electrophoresis. Prevention and control of microbial contamination are required in the future.

Key Findings

Slightly fewer than 50% of initial bacteria were scattered in the air or deposited on the plate, even considering the number of bacteria after wiping.

Originality

Identification of bacteria directly and rapid analysis.

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10.4225/50/58106ee2a9961
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Suggested Citation

Yudai Takahashi, Shinsuke Kato, U Yanagi, Hideaki Nagano, 2016, Decrease in the number of bacteria for nucleic acid extraction and sampling of microbiome from the environment, 7th International Conference on Energy and Environment of Residential Buildings, November 20-24 2016, Brisbane, Australia, viewed 21 January 2017, <http://dx.doi.org/10.4225/50/58106ee2a9961>.

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